The optional 6800-01 Multiphase Flash™ Fluorometer is a Pulse Amplitude Modulated (PAM) fluorometer that provides simultaneous fluorescence and gas exchange measurements over the same 6-cm2 leaf area. It provides both modulated and non-modulated fluorescence signals. The fluorometer can perform induction kinetics over the entire aperture, as well as measure dark- and light-adapted leaf parameters including Fo, Fm, F, Fm’, Fo’. Calculated parameters include Fv, Fv/Fm, Fv’/Fm’, ØPSII, qP, qN, NPQ, and ETR.
The fluorometer supports the Multiphase Flash™ Fluorescence (MPF) protocol to accurately estimate Fm’ at infinite irradiance. This protocol is a novel approach to determine maximum fluorescence yield (Fm’) with a single measure-ment of less than 1 second (Loriaux et al., 2013).